Isotopic Probes of the Enolase Reaction Mechanism

Isotopic probes of the argininosuccinate lyase reaction.

The mechanism of the argininosuccinate lyase reaction has been probed by the measurement of the effects of isotopic substitution at the reaction centers. A primary deuterium isotope effect of 1.0 on both V and V/K is obtained with (2S,3R)-argininosuccinate-3-d, while a primary 15N isotope effect on V/K of 0.9964 +/- 0.0003 is observed. The 15N isotope effect on the equilibrium constant is 1.018...

The Gas Phase Oxidation of Acetaldehyde Reaction Mechanism and Kinetics

The mechanism of the low temperature oxidation of gaseous acetaldehyde was investigated in the temperature range of 1 50-400?°C. The minor, intermediate and major products were identified and measured quantitatively by sampling directly into the ionization chamber of an MS10-C2 mass spectrometer from the reactor. The formation of H2O, CO, CO2, HCOOH, H2, HCHO, CH3COOH and CH3OH as the major pro...

Mechanism of the propionyl carboxylase reaction. II. Isotopic exchange and tracer experiments.

Experiments with bicarbonate-C4 or methylmalonyl coenzyme A-3-C14 and substrate amounts of crystalline propionyl carboxylase (1) demonstrated unequivocally that the over-all reaction involves carboxylation and decarboxylation of the enzyme according to Reactions 1 and 2. Reaction 1 itself had previously been believed (4-6) to consist of two separate steps. However, careful reinvestigation of th...

Isotopic Separation Factor of Slow Discharge Mechanism of Hydrogen Electrode Reaction. Ii

Kinetics and thermodynamics of the esterification reaction according to the Langmuir-Hinshelwood mechanism

In this work, kinetics and thermodynamics of esterification reaction were studied. This research investigated the esterification reaction between methanol and acidified oil catalyzed by sulfonated cation exchange resin and proposed a new rate equation for consideration of kinetics of this reaction according to the Langmuir-Hinshelwood mechanism. Thermodynamics and kinetics parameters were calcu...